International Training on DNA Barcoding of Marine Life

April 16 -21, 2007

National Bureau of Fish Genetic Resources, Lucknow


Programme

15th April, 2007 Sunday 
Arrival of Participants 
16th April, 2007 Monday 
09.30 hrs to 10.00 hrs Registration
10.00 hrs to 11.00 hrs Inaugural Session
  Welcome and opening remarks
  Dr. W S Lakra, Director, NBFGR
  Remarks by Chairperson of IO-COML: Dr. M. Wafar
  Self Introduction by Participants
  Address by DDG(Fy), ICAR : Dr. S. Ayyappan
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 12.15 Presentation on NBFGR : W S Lakra
12.15 hrs to 13.00 hrs Orientation to Working Labs
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 14.45 hrs Interest on Barcoding of Life and Recent Advances: Dirk Steinke
14.45 hrs to 15.30 hrs CSIRO Australia: DNA Barcoding of Fishes/ Crustaceans/Echinoderms: Ms Bronwyn Holmes
15.30 hrs to 16.00 hrs Tea/Coffee
16.00 hrs to 18.00 hrs Sample Processing for DNA Isolation : Demo and Hands-on Practice Contd. : M.S. Verma
20.00 hrs Reception Dinner
Hosed by Aquatic Biodiversity Conservation Society (ABCS)
17 April, 2007 Tuesday 
09.30 hrs to 10.30 hrs Operational Procedures for DNA Barcoding:
Dirk Steinke
10.30 hrs to 11.00 hrs DNA Isolation: Demo and Hands-on Practice :
M.S. Verma/M. Goswami
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 13.00 hrs DNA Isolation : Demo and Hands-on Practice- Contd. : MS Verma
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 15.30 hrs DNA Isolation :
Demo and Hands-on Practice – Contd. :
M.S. Verma
15.30 hrs to 16.00 hrs Tea/Coffee
16.00 hrs to 18.00 hrs Agrose Gel Electrophoresis/DNA Visualization :
M.S. Verma
18th April, 2007 Wednesday 
09.30 hrs to 10.15 hrs Potential Uses of Barcoding in the Identification of Shark Species from Dried Fins : Bronwyn Holmes
10.15 hrs to 11.00 hrs An Appraisal of Polymerase Chain Reaction:
Vindhya Mohindra
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 12.30 hrs PCR Primers and Primer Designing :
K.V. Singh
12.30 hrs to 13.00 hrs Setting PCR Reaction for mtDNA COI : Demo & Hands on practice: K.V. Singh
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 15.30 hrs Demo and Hands-on Practice – Contd. :
K.V. Singh
15.30 hrs to 16.00 hrs Tea/Coffee
16.00 hrs to 18.00 hrs PCR and PCR Product Assessment:
Demo and Hands-on Practice – Contd.
K.V. Singh
19th April, 2007 Thursday 
09.30 hrs to 10.15 hrs An Appraisal of Mitochondrial DNA Markers
A. Gopalakrishnan
10.15 hrs to 11.00 hrs An Appraisal of DNA Sequencing : V. Mohindra
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 13.00 hrs Sequencing PCR : Demo & Hands-on Practice
V. Mohindra
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 15.30 hrs Operating DNA Sequencing Machine : Appraisal & Demo: P. Punia
15.30 hrs to 16.00 hrs Tea
16.00 hrs to 18.00 hrs

Preprocessing PCR Product for Sequencing : Demo : V. Mohindra
20th April, 2007 Friday 
09.30 hrs to 10.15 hrs Preprocessing for Sequencing – contd.:
V. Mohindra
10.15 hrs to 11.00 hrs mtDNA sequencing :
V. Mohindra and MS Verma
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 13.00 hrs mtDNA Sequencing Demo and Practice – Contd.:
V. Mohindra and MS Verma
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 15.00 hrs mtDNA Sequencing : Reading, Quality Evaluation and Processing : V. Mohindra
15.00 hrs to 15.15 hrs Tea / Coffee
15.15 hrs to till close

Mt DNA Sequence Processing Alignment and Barcoding : A Gopalakrishnan and MS Verma
21st April, 2007 Saturday 
09.30 hrs to 10.15 hrs Application of DNA Barcoding in Identification of Fish eggs and Products : Brownyn Holmes
10.15 hrs to 11.00 hrs BOLD and Future Plans: Dirk Steinke
11.00 hrs to 11.30 hrs Tea/Coffee
11.30 hrs to 13.00 hrs Computational Methods and Softwares for Genetic Conclusions form Barcode sequences : Demo & Practice : Dirk Steinke
13.00 hrs to 14.00 hrs Lunch
14.00 hrs to 15.30 hrs Computational Methods and Softwares for Genetic Conclusions form Barcode Sequences : Demo & Practice – Contd. : Dirk Steinke
15.30 hrs to 16.30 hrs Valedictory Session
16.30 hrs to 17.00 hr Tea/Coffee
20.00 hrs Farewell DinnerHosed by Aquatic Biodiversity Conservation Society (ABCS)
22nd April, 2007 Departure of Participants